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Title: How does extracellular pH affect the genetic transformation frequency of calcium treated Escherichia coli (E. coli)?
Subject: Biotechnology Grade level: High School - Grades 10-12 Academic Level: Advanced Project Type: Experimental Cost: Medium Awards: Gold Medal from Calgary Youth Science Fair 5th place in Sanofi-Aventis Biotech Challenge First Place, Canada Wide Virtual Science Fair (2006) Affiliation: Canada Wide Virtual Science Fair Description: The effect of extracellular pH on the frequency of genetic transformation in calcium-treated Escherichia coli was investigated, focusing on whether pH affects transformation frequency through altering components of the proton motive force, membrane potential and pH gradient. E. coli were made competent and transformed with an ampicillin resistance carrying plasmid, in solutions of different pH ranges. Transformants were selected for ampicillin resistance. A comparison of the experimental transformation frequencies to literature values of the magnitude of components of the proton motive force at the same extracellular pH levels indicates that no component of the proton motive force is involved in E. coli transformation Link: http://www.virtualsciencefair.org/2006/sidh6h2/ |
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