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Environmental sciences science fair project:
How does different pH pond levels affect algae cell structure?




Science Fair Project Information
Title: How does different pH pond levels affect algae (spirogyra) cell structure?
Subject: Environmental Sciences
Grade level: Middle School - Grades 7-9
Academic Level: Ordinary
Project Type: Experimental
Cost: Low
Awards:
1st Place, 7th Life Science, PCA Science Fair
2nd Place, 7th Life Science, ACSI Science Fair
Affiliation: Phillipsburg Christian Academy
Year: 2007
Description: A few algae (spirogyra) cultures are grown in bowls filled with pond water with different pH levels. Samples of each bowl are observed under a microscope over time. Changes in cell structure are recorded by drawing a diagram of the algae.
Link: http://www.fellowshipch.org/pcasfnicholas07.html
Short Background

Spirogyra (Green Algae)

Spirogyra is a genus of filamentous green algae of the order Zygnematales, named for the helical or spiral arrangement of the chloroplasts that is diagnostic of the genus. It is commonly found in freshwater areas, and there are more than 400 species of Spirogyra in the world. Spirogyra measures approximately 10 to 100μm in width and may stretch centimeters long.

Spirogyra is unbranched with cylindrical cells connected end to end in long green filaments. The cell wall has two layers: the outer wall is composed of cellulose while the inner wall is of pectin. The cytoplasm forms a thin lining between the cell wall and the large vacuole it surrounds. Chloroplasts are embedded in the peripheral cytoplasm; their numbers are variable (as few as one). The chloroplasts are ribbon shaped, serrated or scalloped, and spirally arranged, resulting in the prominent and characteristic green spiral on each filament. Each chloroplast contains several pyrenoids, centers for the production of starches, appearing as small round bodies.

Spirogyra can reproduce both asexually and sexually. In asexual reproduction, fragmentation takes place, and Spirogyra simply undergoes intercalary mitosis to form new filaments.

Almost all green algae cells have chloroplasts. These contain chlorophylls a and b, giving them a bright green colour (as well as the accessory pigments beta carotene and xanthophylls), and have stacked thylakoids. All green algae have mitochondria with flat cristae. When present, flagella are typically anchored by a cross-shaped system of microtubules, but these are absent among the higher plants and charophytes. Flagella are used to move the organism. Green algae usually have cell walls containing cellulose, and undergo open mitosis without centrioles.

Source: Wikipedia (All text is available under the terms of the GNU Free Documentation License)

For more information (background, pictures, experiments and references):
Algae K-12 Experiments & Background Information
Spirogyra
Green Algae

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