Biochemistry Science Fair Project
Conventional PCR versus real time PCR in detecting DNA


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Project Information
Title: Conventional PCR versus real time PCR in detecting DNA
Subject: Biochemistry
Grade level: High School - Grades 10-12
Academic Level: Advanced
Project Type: Experimental
Cost: High
Awards: Second Place, Canada Wide Virtual Science Fair (2006)
Affiliation: Canada Wide Virtual Science Fair
Description: Reverse-transcription real-time PCR is used to produce copies of a segment of the influenza virus (the matrix gene). The experiment is about changing the concentration of one of the reagents - magnesium chloride - to find out if it is possible to produce DNA products with more efficiency.
Link: www.virtualsciencefair.org...
Background

In molecular biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (Q-PCR/qPCR) or kinetic polymerase chain reaction, is a laboratory technique based on the polymerase chain reaction, which is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of a specific sequence in a DNA sample.

The procedure follows the general principle of polymerase chain reaction; its key feature is that the amplified DNA is quantified as it accumulates in the reaction in real time after each amplification cycle. Two common methods of quantification are the use of fluorescent dyes that intercalate with double-stranded DNA, and modified DNA oligonucleotide probes that fluoresce when hybridized with a complementary DNA.

Frequently, real-time polymerase chain reaction is combined with reverse transcription polymerase chain reaction to quantify low abundance messenger RNA (mRNA), enabling a researcher to quantify relative gene expression at a particular time, or in a particular cell or tissue type.

Although real-time quantitative polymerase chain reaction is sometimes incorrectly abbreviated as RT-PCR, it should not be confused with reverse transcription polymerase chain reaction, also known as RT-PCR.

For More Information: Polymerase Chain Reaction (PCR)

Source: Wikipedia (All text is available under the terms of the Creative Commons Attribution-ShareAlike License)

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